Summary by objectives:
To detect and obtain T-cells that can act as a measure of immune status and therapy against SARS-CoV2 infection. The cooperation of involved centers must make possible to demonstrate the relevance of this key component of immune status.
As specific Objectives:
- To develop methods for general measurement of the T-cell response against SARS-CoV2.
- From monocytes of individuals, to develop DCs (dendritic cells) transfected with mRNAs to allow intracellular expression of SARS-CoV2 antigens without the need of complete and infective viruses; from these DCs in a process associated with the GMP culture system of (CliniMACS Prodigy or similar), to be able to define and stimulate specific T-cell to determine the type of responses or to be used for treatment of some patient, when necessary.
- From the lymphocytes of the individuals themselves, to modify them by gene transduction of mRNA to control their function towards the possibility of eliminating the virus without being able to generate hyperfunctional effects, that are related to adverse effects or to control these hiperinflammatory syndrome by using Tregs.
