Beatriz Rodriguez

de Quiros Plaza, Esther Bernaldo; López-Abente, Jacobo; Camino, Manuela; Gil, Nuria; Panadero, Esther; Campos, Minia; Seoane, Elena; Pion, Marjorie; Correa-Rocha, Rafael. Transplantation: July 2018 – Volume 102 – Issue – p S143 DOI: 10.1097/01.tp.0000542766.40479.8a Abstract Introduction  Heart transplantation is a successful strategy for the treatment of end-stage pediatric heart diseases. In recent years, improved therapies and clinical practice have led to increased graft survival, and consequently, chronic complications secondary to organ transplantation due in part to long-term immunosuppression have also emerged. Allergies and atopic diseases, such as atopic dermatitis (AD) are among these chronic complications. Despite that AD constitutes a frequent… Leer más »Th1/Th2 Imbalance and Early Age to Transplantation is Associated to Atopic Dermatitis in Heart-Transplanted Pediatric Patients.

Esther Bernaldo de Quirós, Manuela Camino, Nuria Gil, Esther Panadero, Constancio Medrano, Juan Miguel Gil-Jaurena, Marjorie Pion, Giovanna Lombardi, Megan K Levings, Lori J West, Rafael Correa-Rocha. Transplantation: July 2018 – Volume 102 – Issue – p S205 DOI: 10.1097/01.tp.0000542859.38902.af Abstract Immune allograft rejection remains the main obstacle to reach successful transplants. Transfer of regulatory T cells (Treg) has acquired growing interest in attempts to prevent rejection. However, the limited number and the differentiated phenotype of Tregs isolated from blood constitute important drawbacks for the effectiveness of this strategy. In collaboration with several teams, we have explored the use of the… Leer más »“First-In-Human” Clinical Trial Employing Adoptive Transfer of Autologous Thymus-Derived Treg Cells (thyTreg) to Prevent Graft Rejection in Heart-Transplanted Children.

Jorge Gallego-Valle, Verónica Astrid Pérez-Fernández, Rafael Correa-Rocha, Marjorie Pion. Int J Mol Sci 2018 Jun 12;19(6):1737. DOI: 10.3390/ijms19061737 Abstract Regulatory B cells (Bregs) participate in auto-tolerance maintenance and immune homeostasis. Despite their impact on many diseases and due to the difficulty to define them, knowledge about their origin and their physiological inducers is still unclear. The incomplete understanding about the generation of Bregs and their limited numbers in periphery make it difficult to develop Breg-based therapy. Therefore, identifying factors that promote their development would allow their ex-vivo production in order to create new immunotherapy. This project aims to test the capacity of several cytokines… Leer más »Generation of Human Breg-Like Phenotype with Regulatory Function In Vitro with Bacteria-Derived Oligodeoxynucleotides.

E Bernaldo de Quiros, E Seoane-Reula, E Alonso-Lebrero, M Pion, R Correa-Rocha. Allergol Immunopathol (Madr)Nov-Dec 2018;46(6):612-618. Epub 2018 May 5. DOI: 10.1016/j.aller.2018.02.002 Abstract Food allergy is a pathological immune reaction that identifies certain harmless food proteins, usually tolerated by the majority of the people, as a threat. The prevalence of these food allergies is increasing worldwide and currently affects 8% of children. Exacerbated reactions to milk, egg and peanut are the most frequent in the pediatric population. It is well known that allergic diseases are a type 2 T-helper (Th2) immune response, characterized by the elevated production of IgE antibodies. However, little is known about… Leer más »The role of regulatory T cells in the acquisition of tolerance to food allergens in children.

Laura Perezabad, Jacobo López-Abente, Elena Alonso-Lebrero, Elena Seoane, Marjorie Pion, Rafael Correa-Rocha. Pediatr Res. 2017 May;81(5):722-730. Epub 2017 Jan 18. DOI: 10.1038/pr.2017.12 Abstract Background:  Cow’s milk protein allergy (CMPA) is the most common food allergy in infants. However, little is known about which specific immune mechanisms are related with the CMPA onset. The objective was to investigate which immune alterations constitute differential factors between allergy and tolerance, and hence could be implicated in the CMPA establishment in infants. Methods:  An extensive analysis of immune subsets, including Treg and cytokine-secreting cells was performed in blood samples from 28 infants younger than 9 mo obtained 1-4 d after the first… Leer más »The establishment of cow’s milk protein allergy in infants is related with a deficit of regulatory T cells (Treg) and vitamin D.

Jacobo López-Abente, Rafael Correa-Rocha, Marjorie Pion. Front Immunol 2016 May 19;7:192. eCollection 2016. DOI: 10.3389/fimmu.2016.00192 Abstract Regulatory T cells (Tregs) play an important role in infections, by modulating host immune responses and avoiding the overreactive immunity that in the case of human immunodeficiency virus (HIV) infection leads to a marked erosion and deregulation of the entire immune system. Therefore, the suppressive function of Treg in HIV-infected patients is critical because of their implication on preventing the immune hyperactivation, even though it could also have a detrimental effect by suppressing HIV-specific immune responses. In recent years, several studies have shown that HIV-1 can directly… Leer más »Functional Mechanisms of Treg in the Context of HIV Infection and the Janus Face of Immune Suppression.

Didiana Jaramillo-Ruiz, Francisco Javier De La Mata, Rafael Gómez, Rafael Correa-Rocha, M Ángeles Muñoz-Fernández. PLoS One. 2016 Jan 19;11(1):e0145760. eCollection 2016. DOI: 10.1371/journal.pone.0145760 Abstract Background: HIV-1 has proved to infect regulatory T cells (Treg) modifying their phenotype and impairing their suppressive capacity. As Treg cells are a crucial component in the preservation of the immune homeostasis, we researched that the antiviral capacity of carboxilan dendrimers prevents the HIV-1 infection of Treg and their effects. The phenotype and suppressive capacity of Treg treated or non-treated with carbosilane dendrimers were studied by flow cytometry. Treated and non-treated Treg from healthy donors were infected with HIV-1NL4.3. The infection of… Leer más »Nanotechnology as a New Therapeutic Approach to Prevent the HIV-Infection of Treg Cells.

Ana Judith Perisé-Barrios, Rafael Correa-Rocha, Susana Alvarez, Maria Angeles Muñoz-Fernandez, Marjorie Pion. AIDS 2014 Oct 23;28(16):2365-74. Abstract Objective:  Patients infected by the HIV type 1 (HIV-1) frequently show a general deregulation of immune system. A direct influence of HIV-1 particles on B-cell activation, proliferation and B-cell phenotype alterations has been recently described. Moreover, expression of activation-induced cytidinedeaminase (AID) mRNA, which is responsible for class switch recombination (CSR) and somatic hypermutation (SHM), was reported to be overexpressed in B cells exposed to HIV-1. Design:  Study of primary human B cells in an in-vitro model. Methods:  In the current study, we evaluated which signalling pathways are… Leer más »HIV-1 induces B-cell activation and class switch recombination via spleen tyrosine kinase and c-Jun N-terminal kinase pathways.

Gema Méndez-Lagares, Didiana Jaramillo-Ruiz, Marjorie Pion, Manuel Leal, M A Muñoz-Fernández, Yolanda M Pacheco, Rafael Correa-Rocha. J Acquir Immune Defic Syndr . 2014 Mar 1;65(3):278-82. DOI: 10.1097/QAI.0000000000000092 Abstract Indexation of regulatory T cells (Treg) to the number of activated T cells constitutes a homeostatic mechanism ensuring that T-cell expansion remains under control. However, immune hyperactivation observed in HIV-infected patients suggests a possible dysfunction of this mechanism. Here we show that the Treg/IL-2-producing cells’ balance is deeply disturbed in viremic HIV-infected patients. We found a lower expression of IL-2 receptor alpha on Treg from viremic patients. We confirmed in vitro that HIV infection of Treg downregulates IL-2 receptor alpha… Leer más »HIV-1 induces B-cell activation and class switch recombination via spleen tyrosine kinase and c-Jun N-terminal kinase pathways.